Recombinant:Article Title: Allosteric Activation of Protein Phosphatase 5 with Small Molecules.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-CDC37 Santa Cruz Biotechnology Cat. #sc-13129 Rabbit anti-p-CDC37Ser13 Cell Signaling Technology Cat. #13248S Mouse-anti-PP5 Santa Cruz Biotechnology Cat. #sc-271816 Rabbit anti-HSF1 Cell Signaling Technology Cat. #4356T Rabbit anti-HSP70 Cell Signaling Technology Cat. #4872S Rabbit anti-HSP40 Cell Signaling Technology Cat. #4872S Mouse anti-HSP27 Santa Cruz Biotechnology Cat. #sc-131321 Mouse anti-GAPDH Proteintech Cat. #60004-1-Ig Goat horseradish peroxidase (HRP)-linked anti-rabbit IgG Thermo Fisher Scientific Cat. #31460 Goat HRP-linked anti-mouse IgG Thermo Fisher Scientific Cat. #31430 Bacterial strains Trans BL21(DE3) Transgen Cat. #CD601-02 Chemicals and recombinant proteins Human PP5 WT This Paper N/A Human PP5 catalytic domain This Paper N/A Human PP5 TPR domain This Paper N/A Human PP5E428A This Paper N/A Human PP5V429A This Paper N/A Human PP5K430A This Paper N/A Human CDC37 This Paper N/A Human HSP90 This Paper N/A SPEC Compound Library Topscience, Shanghai, N/A S56 China IBS Compound Library Topscience, Shanghai, China N/A Chemdiv Compound Library Topscience, Shanghai, China N/A Compound DDO-3733 This Paper N/A Para-nitrophenyl phosphate (pNPP) Bidepharm Cat. #4264-83-9 EZ-Link NHS-Biotin Thermo Fisher Scientific Cat. #20217 Peptide TD8 (TSRMEEVD) SynthBio Cat. #HSA10079- P211101 RPMI 1640 medium Adamas life Cat. #C8016 DMEM High Glucose VivaCell BIOSCIENCES Cat. #C3101-0500 Fetal Bovine Serum VivaCell BIOSCIENCES Cat. #C04001-500 Penicillin/streptomycin (x100) Gibco ThermoFisher Cat. #15140-122 HiScript III qRT SuperMix for qPCR (+gDNA wiper) Vazyme Cat. #R323-01 ChamQ SYBR qPCR master mix (High ROX Premixed) Vazyme Cat. #Q341-02 Trypsin-EDTA Gibco Cat. #25200-056 PBS Adamas life Cat. #C8020 Western & IP Cell Lysis Buffer Beyotime Cat. #P0013 Critical Commercial Assays Cell Counting Kit-8 (CCK-8) Beyotime Cat. #C0039 RNAsimple Total RNA Kit TIANGEN Cat. #DP419 Experimental models: Cell lines HCT116 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences. .. CSTR:19375.09.3101 HUMSCSP5076 S57 PP5-KO HCT116 Cyagen Biosciences STSKO200904CBC1 SW620 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences.
Drug discovery:Article Title: Allosteric Activation of Protein Phosphatase 5 with Small Molecules.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-CDC37 Santa Cruz Biotechnology Cat. #sc-13129 Rabbit anti-p-CDC37Ser13 Cell Signaling Technology Cat. #13248S Mouse-anti-PP5 Santa Cruz Biotechnology Cat. #sc-271816 Rabbit anti-HSF1 Cell Signaling Technology Cat. #4356T Rabbit anti-HSP70 Cell Signaling Technology Cat. #4872S Rabbit anti-HSP40 Cell Signaling Technology Cat. #4872S Mouse anti-HSP27 Santa Cruz Biotechnology Cat. #sc-131321 Mouse anti-GAPDH Proteintech Cat. #60004-1-Ig Goat horseradish peroxidase (HRP)-linked anti-rabbit IgG Thermo Fisher Scientific Cat. #31460 Goat HRP-linked anti-mouse IgG Thermo Fisher Scientific Cat. #31430 Bacterial strains Trans BL21(DE3) Transgen Cat. #CD601-02 Chemicals and recombinant proteins Human PP5 WT This Paper N/A Human PP5 catalytic domain This Paper N/A Human PP5 TPR domain This Paper N/A Human PP5E428A This Paper N/A Human PP5V429A This Paper N/A Human PP5K430A This Paper N/A Human CDC37 This Paper N/A Human HSP90 This Paper N/A SPEC Compound Library Topscience, Shanghai, N/A S56 China IBS Compound Library Topscience, Shanghai, China N/A Chemdiv Compound Library Topscience, Shanghai, China N/A Compound DDO-3733 This Paper N/A Para-nitrophenyl phosphate (pNPP) Bidepharm Cat. #4264-83-9 EZ-Link NHS-Biotin Thermo Fisher Scientific Cat. #20217 Peptide TD8 (TSRMEEVD) SynthBio Cat. #HSA10079- P211101 RPMI 1640 medium Adamas life Cat. #C8016 DMEM High Glucose VivaCell BIOSCIENCES Cat. #C3101-0500 Fetal Bovine Serum VivaCell BIOSCIENCES Cat. #C04001-500 Penicillin/streptomycin (x100) Gibco ThermoFisher Cat. #15140-122 HiScript III qRT SuperMix for qPCR (+gDNA wiper) Vazyme Cat. #R323-01 ChamQ SYBR qPCR master mix (High ROX Premixed) Vazyme Cat. #Q341-02 Trypsin-EDTA Gibco Cat. #25200-056 PBS Adamas life Cat. #C8020 Western & IP Cell Lysis Buffer Beyotime Cat. #P0013 Critical Commercial Assays Cell Counting Kit-8 (CCK-8) Beyotime Cat. #C0039 RNAsimple Total RNA Kit TIANGEN Cat. #DP419 Experimental models: Cell lines HCT116 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences. .. CSTR:19375.09.3101 HUMSCSP5076 S57 PP5-KO HCT116 Cyagen Biosciences STSKO200904CBC1 SW620 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences.
Real-time Polymerase Chain Reaction:Article Title: Allosteric Activation of Protein Phosphatase 5 with Small Molecules.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-CDC37 Santa Cruz Biotechnology Cat. #sc-13129 Rabbit anti-p-CDC37Ser13 Cell Signaling Technology Cat. #13248S Mouse-anti-PP5 Santa Cruz Biotechnology Cat. #sc-271816 Rabbit anti-HSF1 Cell Signaling Technology Cat. #4356T Rabbit anti-HSP70 Cell Signaling Technology Cat. #4872S Rabbit anti-HSP40 Cell Signaling Technology Cat. #4872S Mouse anti-HSP27 Santa Cruz Biotechnology Cat. #sc-131321 Mouse anti-GAPDH Proteintech Cat. #60004-1-Ig Goat horseradish peroxidase (HRP)-linked anti-rabbit IgG Thermo Fisher Scientific Cat. #31460 Goat HRP-linked anti-mouse IgG Thermo Fisher Scientific Cat. #31430 Bacterial strains Trans BL21(DE3) Transgen Cat. #CD601-02 Chemicals and recombinant proteins Human PP5 WT This Paper N/A Human PP5 catalytic domain This Paper N/A Human PP5 TPR domain This Paper N/A Human PP5E428A This Paper N/A Human PP5V429A This Paper N/A Human PP5K430A This Paper N/A Human CDC37 This Paper N/A Human HSP90 This Paper N/A SPEC Compound Library Topscience, Shanghai, N/A S56 China IBS Compound Library Topscience, Shanghai, China N/A Chemdiv Compound Library Topscience, Shanghai, China N/A Compound DDO-3733 This Paper N/A Para-nitrophenyl phosphate (pNPP) Bidepharm Cat. #4264-83-9 EZ-Link NHS-Biotin Thermo Fisher Scientific Cat. #20217 Peptide TD8 (TSRMEEVD) SynthBio Cat. #HSA10079- P211101 RPMI 1640 medium Adamas life Cat. #C8016 DMEM High Glucose VivaCell BIOSCIENCES Cat. #C3101-0500 Fetal Bovine Serum VivaCell BIOSCIENCES Cat. #C04001-500 Penicillin/streptomycin (x100) Gibco ThermoFisher Cat. #15140-122 HiScript III qRT SuperMix for qPCR (+gDNA wiper) Vazyme Cat. #R323-01 ChamQ SYBR qPCR master mix (High ROX Premixed) Vazyme Cat. #Q341-02 Trypsin-EDTA Gibco Cat. #25200-056 PBS Adamas life Cat. #C8020 Western & IP Cell Lysis Buffer Beyotime Cat. #P0013 Critical Commercial Assays Cell Counting Kit-8 (CCK-8) Beyotime Cat. #C0039 RNAsimple Total RNA Kit TIANGEN Cat. #DP419 Experimental models: Cell lines HCT116 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences. .. CSTR:19375.09.3101 HUMSCSP5076 S57 PP5-KO HCT116 Cyagen Biosciences STSKO200904CBC1 SW620 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences.
Western Blot:Article Title: Allosteric Activation of Protein Phosphatase 5 with Small Molecules.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-CDC37 Santa Cruz Biotechnology Cat. #sc-13129 Rabbit anti-p-CDC37Ser13 Cell Signaling Technology Cat. #13248S Mouse-anti-PP5 Santa Cruz Biotechnology Cat. #sc-271816 Rabbit anti-HSF1 Cell Signaling Technology Cat. #4356T Rabbit anti-HSP70 Cell Signaling Technology Cat. #4872S Rabbit anti-HSP40 Cell Signaling Technology Cat. #4872S Mouse anti-HSP27 Santa Cruz Biotechnology Cat. #sc-131321 Mouse anti-GAPDH Proteintech Cat. #60004-1-Ig Goat horseradish peroxidase (HRP)-linked anti-rabbit IgG Thermo Fisher Scientific Cat. #31460 Goat HRP-linked anti-mouse IgG Thermo Fisher Scientific Cat. #31430 Bacterial strains Trans BL21(DE3) Transgen Cat. #CD601-02 Chemicals and recombinant proteins Human PP5 WT This Paper N/A Human PP5 catalytic domain This Paper N/A Human PP5 TPR domain This Paper N/A Human PP5E428A This Paper N/A Human PP5V429A This Paper N/A Human PP5K430A This Paper N/A Human CDC37 This Paper N/A Human HSP90 This Paper N/A SPEC Compound Library Topscience, Shanghai, N/A S56 China IBS Compound Library Topscience, Shanghai, China N/A Chemdiv Compound Library Topscience, Shanghai, China N/A Compound DDO-3733 This Paper N/A Para-nitrophenyl phosphate (pNPP) Bidepharm Cat. #4264-83-9 EZ-Link NHS-Biotin Thermo Fisher Scientific Cat. #20217 Peptide TD8 (TSRMEEVD) SynthBio Cat. #HSA10079- P211101 RPMI 1640 medium Adamas life Cat. #C8016 DMEM High Glucose VivaCell BIOSCIENCES Cat. #C3101-0500 Fetal Bovine Serum VivaCell BIOSCIENCES Cat. #C04001-500 Penicillin/streptomycin (x100) Gibco ThermoFisher Cat. #15140-122 HiScript III qRT SuperMix for qPCR (+gDNA wiper) Vazyme Cat. #R323-01 ChamQ SYBR qPCR master mix (High ROX Premixed) Vazyme Cat. #Q341-02 Trypsin-EDTA Gibco Cat. #25200-056 PBS Adamas life Cat. #C8020 Western & IP Cell Lysis Buffer Beyotime Cat. #P0013 Critical Commercial Assays Cell Counting Kit-8 (CCK-8) Beyotime Cat. #C0039 RNAsimple Total RNA Kit TIANGEN Cat. #DP419 Experimental models: Cell lines HCT116 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences. .. CSTR:19375.09.3101 HUMSCSP5076 S57 PP5-KO HCT116 Cyagen Biosciences STSKO200904CBC1 SW620 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences.
Lysis:Article Title: Allosteric Activation of Protein Phosphatase 5 with Small Molecules.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-CDC37 Santa Cruz Biotechnology Cat. #sc-13129 Rabbit anti-p-CDC37Ser13 Cell Signaling Technology Cat. #13248S Mouse-anti-PP5 Santa Cruz Biotechnology Cat. #sc-271816 Rabbit anti-HSF1 Cell Signaling Technology Cat. #4356T Rabbit anti-HSP70 Cell Signaling Technology Cat. #4872S Rabbit anti-HSP40 Cell Signaling Technology Cat. #4872S Mouse anti-HSP27 Santa Cruz Biotechnology Cat. #sc-131321 Mouse anti-GAPDH Proteintech Cat. #60004-1-Ig Goat horseradish peroxidase (HRP)-linked anti-rabbit IgG Thermo Fisher Scientific Cat. #31460 Goat HRP-linked anti-mouse IgG Thermo Fisher Scientific Cat. #31430 Bacterial strains Trans BL21(DE3) Transgen Cat. #CD601-02 Chemicals and recombinant proteins Human PP5 WT This Paper N/A Human PP5 catalytic domain This Paper N/A Human PP5 TPR domain This Paper N/A Human PP5E428A This Paper N/A Human PP5V429A This Paper N/A Human PP5K430A This Paper N/A Human CDC37 This Paper N/A Human HSP90 This Paper N/A SPEC Compound Library Topscience, Shanghai, N/A S56 China IBS Compound Library Topscience, Shanghai, China N/A Chemdiv Compound Library Topscience, Shanghai, China N/A Compound DDO-3733 This Paper N/A Para-nitrophenyl phosphate (pNPP) Bidepharm Cat. #4264-83-9 EZ-Link NHS-Biotin Thermo Fisher Scientific Cat. #20217 Peptide TD8 (TSRMEEVD) SynthBio Cat. #HSA10079- P211101 RPMI 1640 medium Adamas life Cat. #C8016 DMEM High Glucose VivaCell BIOSCIENCES Cat. #C3101-0500 Fetal Bovine Serum VivaCell BIOSCIENCES Cat. #C04001-500 Penicillin/streptomycin (x100) Gibco ThermoFisher Cat. #15140-122 HiScript III qRT SuperMix for qPCR (+gDNA wiper) Vazyme Cat. #R323-01 ChamQ SYBR qPCR master mix (High ROX Premixed) Vazyme Cat. #Q341-02 Trypsin-EDTA Gibco Cat. #25200-056 PBS Adamas life Cat. #C8020 Western & IP Cell Lysis Buffer Beyotime Cat. #P0013 Critical Commercial Assays Cell Counting Kit-8 (CCK-8) Beyotime Cat. #C0039 RNAsimple Total RNA Kit TIANGEN Cat. #DP419 Experimental models: Cell lines HCT116 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences. .. CSTR:19375.09.3101 HUMSCSP5076 S57 PP5-KO HCT116 Cyagen Biosciences STSKO200904CBC1 SW620 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences.
CCK-8 Assay:Article Title: Allosteric Activation of Protein Phosphatase 5 with Small Molecules.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-CDC37 Santa Cruz Biotechnology Cat. #sc-13129 Rabbit anti-p-CDC37Ser13 Cell Signaling Technology Cat. #13248S Mouse-anti-PP5 Santa Cruz Biotechnology Cat. #sc-271816 Rabbit anti-HSF1 Cell Signaling Technology Cat. #4356T Rabbit anti-HSP70 Cell Signaling Technology Cat. #4872S Rabbit anti-HSP40 Cell Signaling Technology Cat. #4872S Mouse anti-HSP27 Santa Cruz Biotechnology Cat. #sc-131321 Mouse anti-GAPDH Proteintech Cat. #60004-1-Ig Goat horseradish peroxidase (HRP)-linked anti-rabbit IgG Thermo Fisher Scientific Cat. #31460 Goat HRP-linked anti-mouse IgG Thermo Fisher Scientific Cat. #31430 Bacterial strains Trans BL21(DE3) Transgen Cat. #CD601-02 Chemicals and recombinant proteins Human PP5 WT This Paper N/A Human PP5 catalytic domain This Paper N/A Human PP5 TPR domain This Paper N/A Human PP5E428A This Paper N/A Human PP5V429A This Paper N/A Human PP5K430A This Paper N/A Human CDC37 This Paper N/A Human HSP90 This Paper N/A SPEC Compound Library Topscience, Shanghai, N/A S56 China IBS Compound Library Topscience, Shanghai, China N/A Chemdiv Compound Library Topscience, Shanghai, China N/A Compound DDO-3733 This Paper N/A Para-nitrophenyl phosphate (pNPP) Bidepharm Cat. #4264-83-9 EZ-Link NHS-Biotin Thermo Fisher Scientific Cat. #20217 Peptide TD8 (TSRMEEVD) SynthBio Cat. #HSA10079- P211101 RPMI 1640 medium Adamas life Cat. #C8016 DMEM High Glucose VivaCell BIOSCIENCES Cat. #C3101-0500 Fetal Bovine Serum VivaCell BIOSCIENCES Cat. #C04001-500 Penicillin/streptomycin (x100) Gibco ThermoFisher Cat. #15140-122 HiScript III qRT SuperMix for qPCR (+gDNA wiper) Vazyme Cat. #R323-01 ChamQ SYBR qPCR master mix (High ROX Premixed) Vazyme Cat. #Q341-02 Trypsin-EDTA Gibco Cat. #25200-056 PBS Adamas life Cat. #C8020 Western & IP Cell Lysis Buffer Beyotime Cat. #P0013 Critical Commercial Assays Cell Counting Kit-8 (CCK-8) Beyotime Cat. #C0039 RNAsimple Total RNA Kit TIANGEN Cat. #DP419 Experimental models: Cell lines HCT116 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences. .. CSTR:19375.09.3101 HUMSCSP5076 S57 PP5-KO HCT116 Cyagen Biosciences STSKO200904CBC1 SW620 Cell Resource Center, Shanghai Institute of Life Sciences, Chinese Academy of Sciences.
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